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Fluorescent
light or Phosphorescence light measurement
Fluorescence is
the phenomenon in which absorption of light of a given
wavelength by a fluorescent molecule is followed by the emission
of light at longer wavelengths. The distribution of
wavelength-dependent intensity that causes fluorescence is known
as the fluorescence excitation spectrum, and the distribution of
wavelength-dependent intensity of emitted energy is known as the
fluorescence emission spectrum.
Fluorescence
detection has three major advantages over other light-based
investigation methods: high sensitivity, high speed, and safety.
The point of safety refers to the fact that samples are not
affected or destroyed in the process, and no hazardous
byproducts are generated.
Sensitivity is
an important issue because the fluorescence signal is
proportional to the concentration of the substance being
investigated. Relatively small changes in ion concentration in
living cells can have significant physiological effects. Whereas
absorbance measurements can reliably determine concentrations
only as low as several tenths of a micromolar, fluorescence
techniques can accurately measure concentrations one million
times smaller -- pico- and even femtomolar. Quantities less than
an attomole (<10-18 mole) may be detected.
Using
fluorescence, one can monitor very rapid changes in
concentration. Changes in fluorescence intensity on the order of
picoseconds can be detected if necessary.
Because it is a non-invasive technique, fluorescence does not
interfere with a sample. The excitation light levels required to
generate a fluorescence signal are low, reducing the effects of
photo-bleaching, and living tissue can be investigated with no
adverse effects on its natural physiological behavior.
Configuration of FluoroMate FS-2
The FluoroMate
FS-2 is comprised of a Light Source, Excitation and Emission
Monochromators & Detectors and the Sample Compartment. The 150W
Xenon lamp provides sample illumination over a 200nm ~ 900nm
range. The light is split in two by a Beam Splitter located
directly in front of the Sample Compartment. One beam irradiates
the Sample while the remainder is read by a Photodiode detector,
monitoring excitation energy. The light emitted by the Sample is
detected by a PMT detector after passing through the
Monochromator. To ensure higher emission intensity, we use a
specially developed signal amplifying PMT.
Applications
Designed for high precision and
accuracy, the FluoroMate FS-2 ensures superior results and
reliability for a wide range of applications, including:
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Life Sciences
Study of basic biological reactions measuring low volume
samples, polarization/anisotropy.
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Pharmaceuticals and Medicine
Measuring trace levels of antibiotics, providing information on
the structure of nucleic acids, Proteins and viruses.
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Analytical Chemistry
Identification and detection of fluorescent material, solvent
effect, quantum yields and lifetimes, chemical reactions.
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Environmental management
Detection and identification of organic and inorganic toxic
substances in air, water and soil.
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Industry
Nutritional quality in the food industry. Quality of paint,
polymers, optical brighteners and phosphor coatings.
Characterization of crude oils.
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Photo Chemistry
Catheterization of excited states, micelle structure, reaction
kinetics in micelles.
Specifications
- Light source: 150W Xenon lamp
- Wavelength Range: 200 nm ~ 900nm
- Sensitivity: S/N>500 (peak to peak)
- Grating: 1200grooves/250 nm blazing (200nm ~ 900nm
- Wavelength Scan speed: Max. 5000 nm/min
- Wavelength Drive speed: 10,000 nm/min
- Wavelength Accuracy: Within +-2nm
- Wavelength repeatability: Within +-2nm
- Slit width: variable 1nm, 2nm, 5nm, 10nm, 20nm
- Dimensions: 518mm(W)×604mm(D)×272mm(H)
- Weight: 43.7kg
- Power requirement: 100 ~ 132 VAC 50/60 Hz or 180 ~ 240 VAC 50/60
Hz (Selectable)
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